Cell lines are crucial tools in biomedical research, providing reliable models to study diverse aspects of human biology and diseases. These immortalized human or animal cell cultures offer a consistent platform for experiments. However, maintaining the integrity of cell lines is vital for valid research outcomes. Cell Line Authentication tests play a crucial role in proving the genetic identity of cell lines and verifying their purity. Accurate authentication enhances scientific reliability, integrity, and transparency in cell-based research, advancing biology and therapy development.
How Does Cell Line Authentication Work?
Cell line authentication depends on short tandem repeats (STRs), which are repetitive sequences of 3-6 base pairs. These sequences vary among individuals and can repeat in specific patterns.
The ATCC/ANSI/ASN-0002 method uses STR profiling for human cell line authentication. First, the STRs are amplified using polymerase chain reaction (PCR) with a specific sequence.
Finally, this profile is compared to a reference sample, confirming the cell line's origin based on its unique pattern of repeats. The resulting profile is isolated using capillary electrophoresis to determine the number of repetitions in the sample. The profile is then compared with a reference sample to verify the cell line's origin.
Importance of Cell Line Authentication
Cell line authentication is crucial due to several significant reasons:
- Maintaining Scientific Integrity: Authenticating cell lines prevents misrepresentation and strengthens the credibility of scientific research. Authentic results are vital for building upon existing knowledge and advancing scientific understanding.
- Quality Assurance: Maintains the quality and consistency of research findings by avoiding misidentification or contamination of cell lines, protecting the reliability of scientific data.
- Data Reproducibility: Ensures that findings can be replicated accurately, contributing to the credibility of scientific discoveries in the broader scientific community.
- Regulatory Compliance: Meets regulatory requirements in biopharmaceutical & biotechnology industries, ensuring adherence to quality control standards and ethical guidelines in research.
Why Choose DNA Forensics Laboratory Pvt. Ltd. for Cell Line Authentication in India?
DNA Forensics Laboratory Pvt. Ltd. is one of the best and most reputable companies for DNA testing in India and abroad. We offer accurate, reliable, & accredited Cell Line Authentication in India at a competitive cost. Moreover, we are the only Indian private company that offers legal DNA tests for honorable courts of law.
Our highly skilled and technical team used the latest & cutting-edge technology for cell line authentication tests. We have over 400 collection centers for DNA tests in India & abroad. You can visit our nearby collection center to give your DNA sample for testing.
Our customers are from reputable research institutes, pharmaceutical and biotechnological companies, etc. Moreover, we offer cell line authentication test reports in India in 10-15 working days.
To schedule an appointment and for further information about cell line authentication in India, call us at +91 8010177771. You can also chat with us via WhatsApp messages at +91 9213177771.
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